Limited Competitive Bidding (Pre-qualification Type) for Contracted Services for Gazami Microsatellite DNA Analysis in FY2026

Limited Competitive Bidding (Pre-qualification Type) for Contracted Services for Gazami Microsatellite DNA Analysis in FY2026. Announcement We will conduct a limited competitive bidding (pre-qualification type) as follows. October 23, Reiwa 8 Orderer: Director of Saga Prefectural Ariake Fisheries Promotion Center, Norihisa Nakajima 1. Matters Concerning Competitive Bidding (1) Commissioned Work Name: Contracted Services for Gazami Microsatellite DNA Analysis in FY2026. (2) Specifications, etc.: According to the specifications. (3) Delivery Deadline: Friday, March 12, Reiwa 9. (4) Delivery Location: Saga Prefectural Ariake Fisheries Promotion Center (2753-2 Nagata, Ashikari-cho, Ogi City, Saga Prefecture). 2. Matters Concerning Bidding Participation Qualifications Bidders must satisfy all of the following requirements. (1) From April 1, Reiwa 3, to the date of this announcement, the bidder must have concluded contracts for similar work (DNA analysis) ordered by government agencies such as the national government (including independent administrative agencies) and prefectures, and must have faithfully performed all of them through in-house analysis. (2) Not a person who falls under the provisions of Article 167-4 of the Local Autonomy Act (Cabinet Order No. 16 of 1947). (3) Not a person who has filed for commencement of rehabilitation proceedings under the Corporate Reorganization Act (Law No. 154 of 2002) or for commencement of rehabilitation proceedings under the Civil Rehabilitation Act (Law No. 225 of 1999). (4) Not a person who has had a dishonored check or bill at a financial institution, etc. for the period from 6 months before the bid opening date to the bid opening date. (5) Not subject to suspension of designation or suspension of bidding qualification for contracts ordered by Saga Prefecture, or not falling under the "Guidelines for Temporary Suspension of Bidding for Contracts, etc. Ordered by Saga Prefecture". (6) Not a person who, or whose officers, etc., falls under any of the following, and not a person who is substantially involved in the management of any of the persons listed in (a) to (g) below. a. Organized crime group (meaning an organized crime group as defined in Article 2, Item 2 of the Act on Prevention of Unjust Acts by Organized Crime Group Members (Law No. 77 of 1991). The same applies hereinafter). b. Member of an organized crime group (meaning a member of an organized crime group as defined in Article 2, Item 6 of the Act on Prevention of Unjust Acts by Organized Crime Group Members (Law No. 77 of 1991). The same applies hereinafter). c. A person who has not been a member of an organized crime group for 5 years. d. A person who uses an organized crime group or its members for the purpose of unfairly benefiting themselves, their company, or a third party, or for the purpose of causing damage to a third party. e. A person who directly or actively cooperates with or is involved in the maintenance and operation of an organized crime group by providing funds or conveniences to an organized crime group or its members. f. A person who has a socially reprehensible relationship with an organized crime group or its members. g. A person who uses an organized crime group or its members while knowing that they are such. 3. Matters Concerning Bidding Procedures, etc. (1) Contact Person: Saga Prefectural Ariake Fisheries Promotion Center, Resource Research Section, 2753-2 Nagata, Ashikari-cho, Ogi City, Saga Prefecture 849-0313. Tel: 0952-66-2000. Fax: 0952-66-4443. (2) How to Obtain Specifications, etc.: Will be posted on the Saga Prefecture website from Friday, October 23, Reiwa 8, to Tuesday, November 10, Reiwa 8. (3) Bidding Briefing Session: Not held. (4) Confirmation of Bidding Participation Qualifications: i. Bidders who wish to participate in the bid (hereinafter referred to as "Bidders") shall submit the "Bid Participation Qualification Confirmation Application" along with the "Business Overview" and "Similar Work Performance Record" to the department specified in 3.(1) by 5:00 PM on Friday, October 30, Reiwa 8, either in person or by registered mail. ii. Those who fail to submit by the deadline or are deemed ineligible to participate in the bid will not be allowed to participate. iii. If requested to explain the submitted documents, the bidder must comply. Additional documents may be requested as necessary. iv. Submitted documents will not be returned. The submitted documents will not be used for purposes other than those related to the commissioned work. v. The results of the confirmation of bidding participation qualifications will be notified by Thursday, November 5, Reiwa 8. Those deemed ineligible may request an explanation of the reasons in writing to the department specified in 3.(1) by Thursday, November 12, Reiwa 8. (5) Loss of Bidding Participation Qualification: Bidders will lose their qualification if any of the following occurs by the bid submission deadline. i. If an application for provisional seizure, provisional disposition, auction, bankruptcy proceedings, company reorganization, corporate reorganization proceedings, special liquidation, or civil rehabilitation proceedings is filed against the bidder. ii. If there is a fact of transaction suspension by a clearinghouse, suspension of transactions by major business partners, etc., and it is recognized that the bidder's business execution is difficult. iii. If any other event occurs that makes it difficult to commence or proceed with the commissioned work. (6) Deadline, Location, and Method of Bid Submission: i. Deadline: 5:00 PM, Tuesday, November 10, Reiwa 8. ii. Location: Department specified in 3.(1) above. iii. Method of Submission: Mail (registered mail) to the department specified in 3.(1) above. Write "Bid Form for Contracted Services for Gazami Microsatellite DNA Analysis in FY2026" in red on the envelope cover. Bids arriving after the deadline will be invalid and will not be opened. (7) Date and Time and Location of Bid Opening: i. Date and Time: 9:00 AM to, Wednesday, November 11, Reiwa 8. ii. Location: 1st Floor Conference Room, Saga Prefectural Ariake Fisheries Promotion Center. 4. Other (1) Bid Bond and Contract Deposit: i. Bid Bond: A deposit of 5/100 or more of the estimated amount (including consumption tax and local consumption tax) multiplied by the number of samples to be analyzed. However, it will be exempted if it falls under any of the provisions of Article 103, Paragraph 3, Items 1, 2, or 3 of the Saga Prefectural Financial Regulations (Reiwa 4 Saga Prefectural Regulations No. 35). ii. Contract Deposit: A deposit of 10/100 or more of the contract amount (including consumption tax and local consumption tax) multiplied by the number of samples to be analyzed. However, it will be exempted if it falls under any of the provisions of Article 115, Paragraph 3, Items 1, 2, 3, or 4 of the Saga Prefectural Financial Regulations (Reiwa 4 Saga Prefectural Regulations No. 35). (2) Location where Contract Terms are Shown: Same as 3.(1) above. (3) Matters Concerning Bidding Method: The bid amount shall be the cost per sample for analysis, etc. related to this commissioned work. For bid award, the bid price will be the amount written on the bid form plus 10% of that amount (if there is a fraction of less than 1 yen, it will be rounded down). Therefore, bidders shall write the amount equivalent to 100/100 of the estimated contract desired amount on the bid form, regardless of whether they are a taxable vendor or a tax-exempt vendor for consumption tax and local consumption tax. (4) Invalid Bids: Bids submitted by any of the following shall be invalid. i. Those who do not have the qualifications to participate. ii. Those who have engaged in fraudulent acts in the said bid. iii. Those who have submitted bid forms with errors, omissions, or illegible signatures or seals. iv. Those who have submitted bid forms with characters and symbols written in a way that can be easily erased. v. Those who have not written the "\" symbol at the beginning of the bid amount or have not used Arabic numerals for the bid amount. vi. Those who have submitted bid forms with corrected amounts. vii. Those who have submitted bid forms with unclear intentions due to errors or omissions. viii. Those deemed invalid due to error under Article 95 of the Civil Code (Law No. 89 of 1896). ix. Those who have submitted two or more bids. x. In addition to the above, those who have violated the conditions of the bid. (5) Cancellation of Bids, etc.: Bids will be canceled if any of the following apply. Any damages incurred in this case shall be borne by the bidder. i. If it is recognized that fair bidding cannot be executed due to bidders colluding or engaging in disruptive behavior, the said bidders may be prevented from participating in the bid, or the execution of the bid may be postponed or canceled. ii. If the bid or bid opening cannot be conducted due to natural disasters or other unavoidable reasons, the execution of the bid may be postponed or canceled. (6) Method of Determining Successful Bidder: i. The bidder who submitted the lowest valid bid within the limit of the estimated price shall be the successful bidder. ii. If there are two or more bidders with the same lowest bid price, a lottery will be held immediately to determine the successful bidder. The method of lottery will be determined according to the "Method of Lottery for Equal Bids" attached hereto, so please write any 3-digit number in the lottery number field of the bid form. iii. If there is no successful bidder as a result of the first bid opening (if there are no bids within the limit of the estimated price), a second bid will be held, and a new bid date will be notified. iv. The number of bidding rounds shall be limited to three. If there is no successful bidder, negotiations for a discretionary contract may be held with the bidder who submitted the lowest bid among those who submitted valid bids in the final round, in accordance with the provisions of Article 167-2, Paragraph 1, Item 8 of the Local Autonomy Act. FY2026 Gazami Microsatellite DNA Analysis Services Commissioned Work Specifications 1. Analysis Content: DNA extraction from Gazami, microsatellite DNA analysis, and genotype determination. 2. Planned Samples for Analysis, etc.: (1) Number of samples planned for analysis: 1,000-2,000 samples (may increase or decrease depending on catch status). (2) Samples for analysis: Gazami samples (preserved in ethanol) and DNA extraction solution sent by the prefecture. Gazami samples include catches, seedstock (approx. 5-10 mm), and parent crabs used for seedstock production. (3) Sample shipping period: Samples will be shipped in approximately three installments after the contract is concluded. The cost of shipping samples, etc. shall be borne by the contractor. 3. Analysis Method: (1) DNA Extraction: i. Extract DNA from Gazami samples preserved in ethanol, one sample at a time. After extraction, ensure RNA removal by RNase treatment. ii. The DNA extraction solution shall be delivered in 1.5ml micro-tubes containing at least 20μl of a solution with a concentration of 10ng/μl or higher, in addition to being used in (2). (2) For microsatellite DNA, amplify the target region by PCR reaction using the following primers (tail sequences of 7 bases provided by Applied Biosystems are not added), and then measure the size of the amplified product using a DNA sequencer (Applied Biosystems 3730 xl DNA Analyzer). Use GeneScan™ 600 LIZ Size Standard (manufactured by Applied Biosystems) as the size standard. Follow the instructions of the client regarding capillary length and run module. PT69 (Dye VIC) Forward: 5’-TTATAATGTAGGCGCTCGCTGGAC-3’ Reverse: 5’-CCTTCCTCTGCTGCGTCTCGTC-3’ PT720 (Dye NED) Forward: 5’-CAGGGAGGCTGAGACAAGGTGTGT-3’ Reverse: 5’-TCAGTGCGTGGTGTAATCATGTCA-3’ PT322 (Dye PET) Forward: 5’-GCCAGTCCACTAGTCGTACGGTCA-3’ Reverse: 5’-ATGGAGACGTGACCGAGATTGCAT-3’ PT659 (Dye PET) Forward: 5’-TCACGGAAGGACTGAATAGAGACG-3’ Reverse: 5’-TTTCTGGCAGTCCTTTCCTTTCAA-3’ C5 (Dye VIC) Forward: 5’-TGTAGGTAGATAGGTAGGAAGG-3’ Reverse: 5’-ATGAACTGAGCACAGGAG-3’ C6 (Dye FAM) Forward: 5’-CGCTACCTCTACTCATCC-3’ Reverse: 5’-GTGTCTAGTGCGTCCAAC-3’ C13 (Dye FAM) Forward: 5’-CTGTCTGATGAGTGAGGCTAC-3’ Reverse: 5’-TGACCACGAGGAAAGGAG-3’ H11 (Dye NED) Forward: 5’-GCCCTGATACTCGGTGAA-3’ Reverse: 5’-GGAGCAGAGGCAGCAATA-3’ - Genotype determination shall be performed for each sample using analysis software (GeneMapper, manufactured by Applied Biosystems, Inc.). - Confirm whether the size standard waveform is good and the size setting is correct using the separately sent size standard check sheet or any arbitrary format, and deliver the confirmation results as the final product. - Analysis values (allele values) shall be recorded up to the second decimal place. - For any other questions or details, follow the instructions of the client. 4. Final Products: (1) DNA extraction solution: 1 tube per sample (measure the DNA concentration of 10/100 samples with a spectrophotometer and attach the results). However, attach the specified sample number to the delivered micro-tubes in a readable manner. (2) Sequence waveform data (electronic file only): 1 set. (3) Electrophoresis raw data (electronic file with extension ".fsa"): 1 set. (4) Genotype determination results (electronic file only): 1 set. (5) Size standard check sheet (electronic file only): 1 set. Regarding (2) Sequence waveform data, indicate the breakdown of samples within each file in the format specified by the client. Regarding (3) Genotype determination results, present the results in the order of markers: C5, C13, H11, PT659, C6, PT322, PT69, PT720. 5. Delivery Deadline: Friday, March 12, Reiwa 9. 6. Delivery Location: Saga Prefectural Ariake Fisheries Promotion Center (2753-2 Nagata, Ashikari-cho, Ogi City, Saga Prefecture 849-0313). Reference Materials 10×Buffer 1μl 94℃ 1 min dNTPs 1μl 94℃ 30 sec 25mM MgSO4 0.6μl 48℃ 1 min 30 cycles Primers Crab12S-4 0.1μl 72℃ 3 min D-DR 0.1μl 72℃ 7 min KOD-Plus-Ver.2 0.1μl 4 ℃ ∞ DW 6.1μl Sample (1-10ng/μl) 1μl Total 10μl Method of Lottery for Equal Bids (If there are two or more bidders with the same lowest bid price, the successful bidder will be determined by lottery.) If there are two or more bids with the same lowest price as a result of the bid opening for mail-in bids, the successful bidder will be determined by lottery using the following method. Therefore, bidders must write "Lottery Number" on their bid form in preparation for the lottery. (If the "Lottery Number" is not written, it will be "000".)