High-speed cell sorting system

Designation of the device / system: High-speed cell sorting system with 5-laser configuration. 1. Scope of service: Delivery, installation, and commissioning of a fully functional 5-laser high-speed cell sorter, including software, accessories, integration into an S2 safety workbench, training, documentation, and a minimum of 2 years warranty. 2. Functional requirements (mandatory): The system must mandatorily enable: - High-parametric flow cytometry for simultaneous analysis of complex cell populations at the single-cell level (?20 simultaneously measurable parameters including FSC/SSC) - Parallel detection and resolution of weakly expressed fluorescence markers in heterogeneous samples with high spectral separation - Real-time identification and sorting of defined cell subpopulations at the single-cell level - Simultaneous multi-fraction cell sorting into ?4 target populations in a single sorting process (no sequential sorting) - Index sorting with unambiguous assignment of each individual cell to downstream single-cell analysis - Aseptic sorting of sensitive cell types while maintaining high cell viability and integrity - Stable, reproducible analysis and sorting performance even at high event rates and complex multicolor experiments - Standardized and interoperable data output for downstream bioinformatics analysis. Simultaneous detection of multiple weakly expressed markers in heterogeneous samples with high spectral separation. 3. Minimum technical requirements (mandatory): Measuring range / optical configuration: - At least 5 permanently integrated and simultaneously available lasers (355 nm, 405 nm, 488 nm, 561 nm, 640 nm) - Full simultaneous use of all laser lines in regular measurement operation - At least 20 simultaneously and independently detectable parameters (incl. FSC/SSC) - Detection of weak fluorescence signals (<100 MESF FITC, <30 MESF PE) under standard measurement conditions. Accuracy / Resolution: - Sorting purity ? 98% under routine conditions - High reproducibility in complex multicolor and multigrating analyses - Stable signal separation even with spectrally overlapping fluorochromes. Speed / Throughput: - ? 25,000 events/s with active multi-way cell sorting - Simultaneous sorting into at least 4 target populations without sequential switching - No significant impairment of sorting purity or recovery at maximum throughput. Interfaces / Compatibility: - Data export in FCS 3.0 format - Integrated software for acquisition, analysis, and sorting - Compatibility with multiwell plates, PCR tubes, and slides. Environmental conditions / Biosafety: - Operation in a closed sorting environment with aerosol control - Integration into S2 safety workbench (or equivalent) - Temperature-controlled sample handling - Protective measures for personnel, sample, and environment. 4. Optional / desirable requirements: - Automated quality control and performance monitoring - Expandable multicolor panel capability - Automated cleaning and sterilization processes - Extended safety and monitoring functions. 5. Compatibility / Integration (if relevant): The system must be integrable into the existing infrastructure of the Core Facility and must meet the following requirements in particular: a. Compatibility with existing high-performance analysis devices (5-laser systems): - The system must fully support 5-laser standard configurations (355 nm, 405 nm, 488 nm, 561 nm, 640 nm). - The optical detection channels must allow the transfer of existing multicolor panels without changing the basic fluorochrome selection. - The spectral detection ranges must be compatible with established 5-laser analysis platforms, so that identical fluorochromes can be measured under comparable excitation and detection conditions. - The system must allow the use of existing compensation and panel designs without structural...